Premium
In vitro modulation of extracellular matrix genes by stingless bee honey in cellular aging of human dermal fibroblast cells
Author(s) -
Abdul Malik Nurfairuz,
Mohamed Mahaneem,
Mustafa Mohd Zulkifli,
Zainuddin Azalina
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13098
Subject(s) - stingless bee , fibroblast , extracellular matrix , senescence , matrix metalloproteinase , microbiology and biotechnology , gene expression , dermal fibroblast , biology , honey bee , matrix (chemical analysis) , in vitro , gene , chemistry , biochemistry , botany , hymenoptera , chromatography , apidae
This study determined the antiaging effect of stingless bee honey on the expression of extracellular matrix genes. MTS (3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt) assay was performed for determination of optimum concentration and incubation time of stingless bee honey. Gene expression of matrix metalloproteinase‐1 (MMP‐1) and collagen type Ⅰ (COL1A1) were analyzed using real time reverse transcriptase polymerase chain reaction technique. Incubation with stingless bee honey at concentration of 0.02% for 72 hr showed significant increase in the viability of human fibroblast cells. Stingless bee honey significantly downregulates metalloproteinase‐1 gene expression in both pre‐senescence and senescence fibroblast cells and upregulates collagen type Ⅰ gene expression in senescence fibroblast cells. In conclusion, stingless bee honey potentially delayed skin aging through modulation of extracellular matrix genes. Practical applications Changes of the extracellular matrix regulation promote skin aging. Stingless bee honey is a good source of natural antioxidant which potentially delays skin aging. This study demonstrated that stingless bee honey beneficially increases collagen type Ⅰ expression and decreases MMP‐1 expression during cellular aging of human dermal fibroblast cells.