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Preparation of dendritic polymer‐based magnetic carrier for application of bromelain separation and purification
Author(s) -
Luo Peng,
Han Juan,
Li Yuan,
Wang Yun,
Wang Li,
Ni Liang
Publication year - 2019
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12976
Subject(s) - bromelain , adsorption , chromatography , magnetic separation , chemistry , elution , materials science , chemical engineering , organic chemistry , protease , engineering , metallurgy , enzyme
Bromelain has wide applications in different industries, such as food, textile, and medicine. Traditional approaches for bromelain separation and purification from solution still have many problems, including unsatisfactory binding efficiency, time‐consuming operation, and costly equipment. In the present study, a new type of dendritic polymer‐based magnetic carrier (GO@Fe 3 O 4 @PEI‐Cu 2+ ) was first prepared for bromelain separation and purification in solution. The histidine existing in bromelain could bind to Cu 2+ cations adsorbed on the surface of the magnetic carrier, and the magnetic carrier showed excellent performance for bromelain separation and purification in solution, with the adsorption capacity up to 357 mg/g. The magnetic carrier also exhibited excellent property in the aspect of recyclability. It was found that the magnetic carrier also presented desirable performance for the separation and purification of bromelain from the crude extract of pineapple peel, and the bromelain structure remained intact before and after elution process. Practical applications Considering many advantages of bromelain in the applications of pharmaceutical and food industries, this study is aimed at presenting a novel magnetic carrier with high stability and fabulous performance for bromelain separation and purification in solution and achieving the practical application that the magnetic carrier can efficiently separate bromelain from the crude extract of pineapple peel.