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Spondias mombim L. (Anacardiaceae): Chemical fingerprints, inhibitory activities, and molecular docking on key enzymes relevant to erectile dysfunction and Alzheimer’s diseases
Author(s) -
Ojo Oluwafemi Adeleke,
Afon Adeola Agnes,
Ojo Adebola Busola,
Ajiboye Basiru Olaitan,
Okesola Mary Abiola,
Aruleba Raphael Taiwo,
Adekiya Tayo Alex,
Oyinloye Babatunji Emmanuel
Publication year - 2019
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12772
Subject(s) - chemistry , chlorogenic acid , pharmacology , biochemistry , antioxidant , tyrosinase , enzyme , butyrylcholinesterase , food science , medicine , acetylcholinesterase , aché
Due to the exceptional wide range in biochemical activities of natural plant products, Spondias mombim L. are attaining a new height because they present great prospects for drug advancement. This research was designed to analyze the pharmaceutical properties of S. mombim L. ethyl acetate fraction (SMEAF) on key enzymes relevant to erectile and cognitive dysfunction. SMEAF inhibitory activities of the specified enzymes were determined spectrophotometrically. Chemical profile of SMEAF were assessed by HPLC/MS analysis. Thereafter, molecular docking of the studied enzymes with chlorogenic acid, lutein, and zeaxanthin were carried out using PATCHDOCK. SMEAF had remarkable enzyme inhibitory effects against phosphodiesterase‐5 (PDE‐5), arginase, angiotensin I‐converting enzyme (ACE), cholinesterase, monoamine oxidase A (MAO), ecto‐5′ nucleotidase (E‐NTDase), tyrosinase, and stimulated sodium–potassium ATPase (Na+/K+‐ATPase) activities. HPLC/MS analysis revealed that phenolics and carotenoids were major components in these fraction notably, chlorogenic acid, lutein, and zeaxanthin. Our results suggested that SMEAF could be explored as phytopharmaceuticals. Practical applications Spondias mombim L. are cooked as green vegetable with enormous medicinal value probably due to its polyphenols with potent antioxidant activity. Furthermore, the leaves could also be useful for therapeutic purposes against erectile dysfunction and central nervous system disorders.