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Effects of drying on cholinesterases and angiotensin‐I converting enzyme inhibitory potential and phenolic constituents of African mistletoe ( Loranthus bengwensis L ) leaves from kolanut host tree
Author(s) -
Omojokun Olasunkanmi S.,
Oboh Ganiyu,
Ademiluyi Adedayo O.
Publication year - 2018
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12510
Subject(s) - caffeic acid , chemistry , antioxidant , butyrylcholinesterase , acetylcholinesterase , aché , ziziphus , angiotensin converting enzyme , enzyme , botany , biochemistry , biology , blood pressure , endocrinology
This study investigated the most appropriate drying method (sun drying, oven drying, and shade drying) for mistletoe leaves obtained from kolanut tree. The phenolic constituents were characterized using high performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on cholinesterases and angiotensin‐I converting enzyme (ACE) and antioxidant activities were determined in vitro. The extracts inhibited acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ACE in dose‐dependent manner. However, extract from sun‐dried sample exhibited the highest AChE, BChE, and ACE inhibitory effect while extract from shade‐dried sample had the least. Likewise, sun‐dried sample exhibited the highest antioxidant properties as exemplified by Fe 2+ ‐chelating, 1,1‐diphenyl‐2 picrylhdrazyl, OH, and nitric oxide radical scavenging abilities. This study also revealed the presence of 20 phenolic compounds with caffeic acid being the most predominant. Conclusively, kolanut host tree mistletoe leaves can be used as therapeutic agent in the management of Alzheimer's disease and hypertension. Practical application The best drying method for mistletoe harvested from kolanut host tree was proposed in this study. Having identified the best drying method that retained phenolic constituents, antioxidant activities, anticholinesterase, and antihypertensive potential of kolanut host tree mistletoe leaves, this work maybe applicable in pharmaceutical industry for development of anticholinesterase and antihypertensive drugs with little or no side effect as well as in the food industry as complementary functional food/medicine in the management of Alzheimer's disease and hypertension.