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Reduction of PM2.5 toxicity on human alveolar epithelial cells A549 by tea polyphenols
Author(s) -
Zhang Ying,
Darland Diane,
He Yan,
Yang Lixue,
Dong Xinfeng,
Chang Yanzhong
Publication year - 2018
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12496
Subject(s) - oxidative stress , polyphenol , reactive oxygen species , a549 cell , malondialdehyde , toxicity , pharmacology , superoxide dismutase , chemistry , viability assay , apoptosis , lipid peroxidation , antioxidant , biochemistry , biology , organic chemistry
Tea‐derived polyphenols have anticancer and antioxidant properties, and they can regulate oxidative stress. This study was designed to quantify both the toxic effects of fine particulate matter with aerodynamic diameter less than 2.5 μm (PM2.5) and determine whether tea polyphenols could provide a protective effect against PM2.5 toxicity on human alveolar epithelial A549 cells in vitro. Cytotoxic effects of the PM2.5 on A549 cells were measured by means of cell viability, the expression of caspase‐3, bax/bcl‐2, and C/EBP‐homologous protein (CHOP), and the generation of intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and superoxide dismutase (SOD). The results showed that tea polyphenols ameliorated some of the adverse effects of PM2.5 on A549 cell viability and SOD levels. In addition, tea polyphenols decreased the production of ROS, MDA generation, and apoptosis in response to PM2.5 exposure. Therefore, our results support a role for tea polyphenols in reducing the toxicity of PM2.5, particularly with regard to targeting oxidative stress and apoptosis. Practical applications PM2.5 poses an ongoing threat to human health as a significant environmental pollutant, regionally and globally. We sought to test the ability of a known antioxidant, tea polyphenols, for their ability to ameliorate PM2.5 toxicity in human lung cells in vitro. Our data revealed the benefits of tea polyphenols in attenuating the effects of PM2.5 treatment using several metrics of cellular toxicity, including indicators of oxidative stress and apoptosis. Therefore, our results identify an opportunity for developing new methods to treat PM2.5‐induced toxicity, particularly with regard to designing postexposure intervention to alleviate lung injury.