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Involvement of bacterial quorum sensing signals in spoilage potential of Aeromonas veronii bv. veronii isolated from fermented surimi
Author(s) -
Zhao Dandan,
Lyu Fei,
Liu Shulai,
Zhang Jianyou,
Ding Yuting,
Chen Wenxuan,
Zhou Xuxia
Publication year - 2018
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12487
Subject(s) - quorum sensing , food spoilage , homoserine , aeromonas veronii , microbiology and biotechnology , chromobacterium violaceum , fermentation , aeromonas , food science , biology , biofilm , bacteria , chemistry , genetics
This study characterized the spoilage potential of the N ‐acyl homoserine lactone (AHL)‐producing strain, A eromonas veronii bv. veronii , isolated from fermented surimi, and investigated the effects of exogenous AHLs on its spoilage characteristics. The strain showed a strong ability of producing total volatile base nitrogen (TVB‐N) and putrescine, and four types of AHLs, including N ‐butanoyl‐L‐homoserine lactone (C4‐HSL), N ‐hexanoyl‐L‐homoserine lactone (C6‐HSL), N ‐heptanoyl‐L‐homoserine lactone (C7‐HSL), and N ‐octanoyl‐L‐homoserine lactone (C8‐HSL), were identified from the supernatant by LC–MS/MS. Concentrations of AHLs reached the maximum value as the cells entered stationary phase contents and then decreased rapidly with the increasing pH. C8‐HSL was found to be dominant with contents of 1,536.33 ng/mL and exogenous C8‐HSL was most effective in promoting bacterial growth, biofilm formation, swimming motility, production of TVB‐N and putrescine, and relative genes expression. The results indicated the involvement of AHL‐based quorum sensing system in the regulation of spoilage potential of Aeromonas spp. Practical applications The results of the present study found that Aeromonas veronii bv. veronii was capable of producing four types of AHLs, which were considered to be involved in the regulation of spoilage potential of the strain. This would enrich the understanding of quorum sensing system in Aeromonas and give clue for new strategy of controlling food spoilage in food industry.