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Inhibitory effect of ethanolic extract of Ramulus mori on adipogenic differentiation of 3T3‐L1 cells and their antioxidant activity
Author(s) -
Hwang Seonwook,
Kim JeongKeun,
Kim InHo,
Lim YoungHee
Publication year - 2018
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12469
Subject(s) - adipogenesis , 3t3 l1 , antioxidant , chemistry , peroxisome , lipid droplet , biochemistry , pharmacology , receptor , in vitro , biology
Ramulus mori is known as a medical herb for improving health. The antioxidant and anti‐obesity effects of an ethanolic extract of R. mori containing oxyresveratrol (ERM) were investigated. The antioxidant activity of ERM was determined by in vitro chemical‐ and cell‐based methods. ERM showed high antioxidant activities. In particular, ERM showed a high scavenging activity for peroxyl radicals in both chemical‐ and cell‐based assays. To investigate the inhibitory effect of ERM on adipogenic differentiation, 3T3‐L1 cells were treated with ERM (20, 30, and 40 μg/mL) for 8 days during induction of differentiation. ERM significantly suppressed lipid accumulation in 3T3‐L1 adipocytes and decreased expression levels of adipogenic genes peroxisome proliferator‐activated receptor gamma (PPARγ), CCAAT/enhancer‐binding proteins alpha (C/EBPα), and sterol regulatory element‐binding protein 1 (SREBP1). ERM also induced G 0 /G 1 phase arrest in 3T3‐L1 adipocytes during mitotic clonal expansion (MCE). The results suggest that ERM possesses anti‐adipogenic activity and antioxidant property. Practical applications Ramulus mori , a branch of Morus alba L., has been used as a traditional herb tea in Asia and is approved as a food source in South Korea, indicating it is safe. An ethanolic extract of R. mori containing oxyresveratrol (ERM) significantly suppresses lipid accumulation in 3T3‐L1 adipocytes and inhibits adipogenesis. ERM shows a strong antioxidative activity. Adipogenesis is the process of adipocyte differentiation, which leads to lipid accumulation in obesity. Obesity is one of metabolic disorders and a major risk factor for various chronic diseases. Additionally, antioxidants can protect cells from damage caused by free radicals. Therefore, ERM could be developed as a health functional food to prevent obesity and many diseases caused by oxidative damage.