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Sialoglycoprotein isolated from Carassius auratus eggs promotes osteoblast differentiation via targeting the p38 mitogen‐activated protein kinase‐dependent Wnt/β‐catenin and BMP2/Smads pathways
Author(s) -
Mao Lei,
Wang Na,
Wang Meiling,
Xia Guanghua,
Yu Zhe,
Wang Jingfeng,
Xue Changhu
Publication year - 2018
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12465
Subject(s) - wnt signaling pathway , bone morphogenetic protein 2 , osteoblast , microbiology and biotechnology , bone morphogenetic protein , chemistry , signal transduction , cellular differentiation , catenin , sialoglycoprotein , biology , in vitro , biochemistry , cell , gene
In the current study, sialoglycoprotein isolated from eggs of Carassius auratus promoted MC3T3‐E1 cells proliferation and differentiation, as assessed by MTT, mineralized nodule formation in vitro, as well as new osteoid formation in neonatal mouse calvarias ex vivo. Further research revealed that Ca‐ SGP facilitated osteogenesis via activating BMP2/Smads, Wnt/β‐catenin, and p38MAPK signaling pathways. What is more, p38MAPK inhibitor SB203580 down‐regulated related mRNA and protein expression of BMP2/Smads and Wnt/β‐catenin signaling pathways, suggesting that p38MAPK pathway might be important for activation of BMP2/Smads and Wnt/β‐catenin pathways in Ca‐ SGP‐induced osteoblastic differentiation. In conclusion, it was demonstrated that Ca ‐SGP promotes osteoblasts differentiation via activating p38MAPK‐dependent BMP2/Smads and Wnt/β‐catenin signaling pathways, which may provide basis for the use of Ca ‐SGP as a potential agent to treat bone loss‐associated diseases such as osteoporosis. Practical application Carassius auratus eggs are one of the major by‐products during fish processing and contain sialoglycoprotein which plays an important role in biological functions. However, they have not been high‐value utilized. This study demonstrated that Sialoglycoprotein isolated from eggs of C. auratus ( Ca ‐SGP) promoted MC3T3‐E1 cells proliferation, differentiation and mineralized nodule formation in vitro, as well as neonatal mouse calvarias formation ex vivo, which may provide basis for a novel application of C. auratus eggs as a functional food used to accelerate bone formation.