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Anti‐amyloidogenic effects of Perilla frutescens var. acuta on beta‐amyloid aggregation and disaggregation
Author(s) -
Kim DaJeong,
Kim MinSuk,
Kim Sunggun,
Hwang KwangWoo,
Park SoYoung
Publication year - 2017
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12393
Subject(s) - perilla frutescens , chemistry , ic50 , perilla , amyloid beta , fraction (chemistry) , hexane , biochemistry , pharmacology , chromatography , in vitro , biology , peptide , organic chemistry , raw material
Beta‐amyloid (Aβ) aggregates are a major cause of Alzheimer's disease (AD), and the inhibition of Aβ aggregation is a good therapeutic target against AD. Thus, the effects of Perilla extract (4–100 µg/mL) on the aggregation of Aβ (20 µM) monomers and disaggregation of pre‐aggregated Aβ aggregates were determined. The Perilla methanol extract and its hexane fraction significantly reduced Aβ aggregation determined by a Theoflavin T fluorescence assay (IC 50 = 24.5 and 19.8 µg/mL, respectively) and confirmed by Western blot analysis with native gels. The inhibition of Aβ aggregation by hexane fraction of Perilla extract (20 and 100 µg/mL) rescued the PC12 cells from Aβ aggregate‐induced toxicity to the levels of DMSO‐treated control groups. Furthermore, hexane fraction efficiently disaggregated the preformed Aβ aggregates (IC 50 = 10.2 µg/mL). Perilla frutescens var. acuta leaves exhibited anti‐amyloidogenic effects against Aβ aggregation and disaggregation. Practical application These results suggest that P. frutescens var. acuta has the potential to be developed as a preventive and therapeutic agent.