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Gui‐ling‐gao inhibits C oncanavalin A ‐induced inflammation by suppressing the expressions of i NOS and proinflammatory cytokines in mice isolated splenocytes
Author(s) -
Zhang Huan,
Wu MinYi,
Guo DeJian,
Wan ChunWai,
Chan ShunWan
Publication year - 2017
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12367
Subject(s) - proinflammatory cytokine , splenocyte , concanavalin a , immune system , tumor necrosis factor alpha , inflammation , immunology , nitric oxide synthase , nitric oxide , interleukin , interleukin 10 , downregulation and upregulation , biology , cytokine , endocrinology , biochemistry , gene , in vitro
Abstract Gui‐ling‐gao (GLG), also known as turtle/tortoise jelly, has been traditionally used as a functional food in southern China, Singapore, and Malaysia to regulate the immune system. In the present study, the antiinflammatory effects of GLG against Concanavalin A (Con A)‐induced inflammation in BALB/c mice isolated splenocytes were evaluated. GLG significantly ( p < .05) inhibited Con A‐induced splenocyte proliferation in a concentration‐dependent manner. Con A‐mediated increases in mRNA and protein expressions of inducible nitric oxide synthase (iNOS) and tumor necrosis factor‐alpha (TNF‐α) were suppressed by GLG treatment ( p < .001). GLG decreased the upregulated mRNA expressions of interleukin‐1β (IL‐1β) ( p < .05) and IL‐15 ( p < .001) induced by Con A. These findings suggest that GLG possesses potential antiinflammatory effects by inhibiting the expressions of iNOS and proinflammatory cytokines, such as TNF‐α, IL‐1β, and IL‐15. Practical applications The present study revealed that Gui‐ling‐gao (GLG) exhibited potential antiinflammatory effect. Thus, GLG could be employed as an effective health food for regulating immune system to cope with inflammation.