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Protoaphin‐ fb , Newly Isolated from Galla Rhois , Suppresses LPS‐Stimulated Inflammatory Reactions in Murine Macrophages
Author(s) -
Lee Hee Won,
Kim Yoon Hee
Publication year - 2017
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12317
Subject(s) - lipopolysaccharide , nitric oxide , inflammation , tumor necrosis factor alpha , nitric oxide synthase , chemistry , anti inflammatory , pharmacology , prostaglandin e2 , cytosol , cyclooxygenase , immunology , medicine , biochemistry , enzyme , organic chemistry
Protoaphin‐ fb is a phenolic compound newly isolated from Galla Rhois , a Korean medical plant. In the present study, the anti‐inflammatory effects of protoaphin‐ fb in lipopolysaccharide (LPS)‐stimulated murine macrophages (RAW264.7 cells) were investigated. Protoaphin‐ fb inhibited release of nitric oxide (NO) and prostaglandin E 2 by reducing protein expression levels of inducible NO synthase and cyclooxygenase‐2 in LPS‐treated RAW264.7 cells. Furthermore, protoaphin‐ fb suppressed release of interleukin‐6 and tumor necrosis factor‐α induced by LPS. Further study indicated that protoaphin‐ fb blocked translocation of the p65 subunit of nuclear factor‐κB from the cytosol to nucleus, which is one of the underlying mechanisms of the anti‐inflammatory action of protoaphin‐ fb . Collectively, these data suggest that protoaphin‐ fb might be useful for the treatment of inflammatory diseases. Practicl Applications Galla Rhois has long been used in Korean traditional medicine due to its various pharmacological effects, including hepatic protective effect, anti‐bacterial activity, and anti‐platelet effect. The results of this research demonstrate that protoaphin‐ fb is effective in attenuating inflammation. This result suggests its potential applicability in functional foods, cosmetic industry, and medicine manufacturing for people suffering from inflammation and inflammatory‐related diseases.