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HPLC Analysis of Twelve Compounds from Artemisia sacrorum Ledeb and their Structural Elucidation
Author(s) -
Bao Wugedunqiqige,
Wang Qinghu,
Han Jingjing,
Han Narenchaoketu
Publication year - 2017
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12291
Subject(s) - repeatability , chromatography , chemistry , absorbance , elution , acetonitrile , ethyl acetate , gradient elution , high performance liquid chromatography , chromatographic separation , reproducibility
An investigation of ethyl acetate extracts from Artemisa sacrorum Ledeb ( A. sacrorum ) has led to the isolation of two new flavonoids together with 10 known compounds. At the same time, a high‐performance liquid chromatographic method is described for the simultaneous determination of the 12 compounds in A. sacrorum . The separation by gradient elution was performed on Hypersil ODS‐2 column (250 mm × 4.6 mm, 5 μm) at 30C with acetonitrile and water as the mobile phase, and monitored by absorbance at 261 nm. The recovery of the method is from 93.33 to 98.67%, and linearity ( r > 0.9992) was obtained for 12 compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also achieved. This assay was successfully applied to the determination of 12 compounds in 10 samples. Practical Applications The antitumor effects of A. sacrorum have been well known in folk medicine, which suggesting that extracted components from A. sacrorum could potentially treat tumor disease. Therefore, a detailed analysis and recognition of structural compounds diversity in A. sacrorum is of great importance to evaluate their antitumor effects.