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Assessment the Protective Role of Quercetin on Acrylamide‐Induced Oxidative Stress in Rats
Author(s) -
ElBeltagi Hossam S.,
Ahmed Mahgoub M.
Publication year - 2016
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12262
Subject(s) - malondialdehyde , chemistry , oxidative stress , glutathione peroxidase , acrylamide , glutathione , quercetin , endocrinology , creatinine , lipid peroxidation , medicine , biochemistry , superoxide dismutase , antioxidant , enzyme , organic chemistry , copolymer , polymer
This study aimed to elucidate whether quercetin treatment could modulate acrylamide (ACR)‐induced DNA damage and oxidative changes in rat brain, liver, kidneys and testes tissues. Fifty adult albino rats were divided into five groups. The first group served as normal control, second group received 50 mg/kg quercetin (QTN) and third group received 20 mg/kg ACR. Fourth and fifth groups received dose of ACR along with 25 or 50 mg/kg QTN, respectively. ACR and QTN were given by oral administrations for 30 days. The results showed that, ACR administration induced significant elevation of alanine transferase, aspartate transferase activities, urea, creatinine and Malondialdehyde levels in serum, whereas, Acetylcholine esterase and testosterone levels were reduced after ACR administration. Moreover, ACR treatment increased Glutathione‐S‐transferase, Myeloperoxidase, Glutathione peroxidase activity, 8‐hydroxy deoxyguanosine, tumor necrosis factor‐α and nitric oxide contents in all tissues. QTN significantly improved the previous parameters. It played a role in ameliorating toxic effects of ACR in rats by reducing oxidative stress. Practical Applications Acrylamide was found in various fried, deep fried and oven‐baked foods that are regularly consumed like chips, crisps and bread, also biscuits, crackers and breakfast cereals. Acrylamide exposure led to increase of alanine transferase, aspartate transferase activities, urea, creatinine and Malondialdehyde levels in serum, whereas, Acetylcholine esterase and testosterone levels were reduced. Moreover, ACR treatment increased Glutathione‐S‐transferase, Myeloperoxidase, Glutathione peroxidase activity, 8‐hydroxy deoxyguanosine, tumor necrosis factor‐α and nitric oxide contents in all tissues. Our study revealed the protective role of quercetin on acrylamide‐induced oxidative stress in rats. Quercetin regulate the generation of inflammatory markers and increasing antioxidant enzyme activity in rat liver, kidneys, brain and testes tissues.