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Subacute Effects of Standardized F umaria Vaillantii Lois. Ethanol Extract on Trace Element Levels, Biochemical and Histopathological Parameters in Experimental Liver Toxicity
Author(s) -
Deliorman Orhan Didem,
Hartevioğlu Ali,
Orhan Nilüfer,
Berkkan Aysel,
Gökbulut Alper,
Günhan Ömer,
Pekcan Mert
Publication year - 2016
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12208
Subject(s) - chlorogenic acid , rutin , chemistry , toxicity , catalase , liver toxicity , liver injury , caffeic acid , ethanol , traditional medicine , malondialdehyde , pharmacology , antioxidant , food science , biochemistry , medicine , organic chemistry
In this study, the hepatoprotective activity of ethanol extracts of F umaria vaillantii Lois. was investigated against CC l 4 ‐induced toxicity in rats. F . vaillantii (500 mg/kg) caused a significant decrease in the plasma aspartate aminotransferase (AST), alanine aminotransaminase (ALT) and tissue malondialdehyde levels, and a significant increase in the reduced glutathione and catalase levels, compared with the CC l 4 group. These findings were approved by histopathological observations. Additionally, a significant decrease was found in liver C u levels (18.5%) of the extract‐treated group (500 mg/kg), while an increase in liver Z n (16.2%) levels was observed compared with the CC l 4 group. These changes in liver Z n and C u levels caused by F . vaillantii extract may contribute to its positive effects on liver toxicity. Phenolic acids and flavonoids in the extract were examined and the extract was standardized by reversed‐phase high‐performance liquid chromatography with diode array detection on the basis of rutin, chlorogenic and caffeic acids as markers. Practical Applications F umaria species have been used both as food and traditional medicine. In some M editerranean countries, the leaves of the plant are cooked or eaten as salad. In T urkey, they are also being drunk as herbal tea, eaten as salad and roasted. The results suggested that F . vaillantii ethanol extract standardized by rutin, chlorogenic and caffeic acids had a significant protective effect against CC l 4 ‐induced hepatotoxicity. Therefore, F . vaillantii can be consumed safely and because of its beneficial effect on liver toxicity, its standardized ethanol extract can be used in the preparation of herbal medicines or food supplements in pharmaceutical and food industry.