Anti‐Glycation of Active Compounds Purified from G raptopetalum Paraguayense

G raptopetalum paraguayense was shown to exhibit antioxidant, hepatoprotective and anti‐hepatocellular carcinoma activities in a recent study. In this work, fractions of the G . paraguayense aqueous solution were extracted using n ‐hexane, ethyl acetate ( EF ) and n ‐butanol, and its antioxidative and anti‐glycative activities were evaluated. The EF extract exhibited the highest antioxidative activity, potently inhibited the formation of advanced glycation end products ( AGEs ) and reduced glycation‐induced protein oxidation. Additionally, the EF extract protected H ep G 2 cells against AGE ‐induced DNA damage. Five major components were isolated and identified in the EF fraction by semi‐preparative high‐performance liquid chromatography, liquid chromatography‐tandem mass spectroscopy and nuclear magnetic resonance: gallic acid, isoquercitrin‐6‐(3‐hydroxy‐3‐methylglutarate), astragalin‐6‐(3‐hydroxy‐3‐methylglutarate), isoquercitrin‐2‐acetyl‐6‐(3‐hydroxy‐3‐methylglutarate) and astragalin‐2‐acetyl‐6‐(3‐hydroxy‐3‐methylglutarate). G . paraguayense exhibited antioxidant and anti‐glycation activities, likely because of the presence of gallic acid. The findings indicate the potential for using G . paraguayense for development of novel treatments for diabetes. Practical Applications Herbal treatments for hyperlipidemia are relatively cheap and locally available. The results indicated that the extracts of G raptopetalum paraguayense may be developed as functional foods for antidiabetic treatment mediated by attenuations of advanced glycation end product oxidation.