Evaluation of In Vitro Invertase Inhibitory Activity of M anilkara zapota Seeds – A Novel Strategy to Manage Diabetes Mellitus

A rtocarpus heterophyllus (jack fruit), M anilkara zapota (sapota), M angifera indica (mango), V itis vinifera (grapes), C itrus sinensis (orange) and S yzygium cumini (jambul) seeds were selected to evaluate the in vitro invertase inhibitory activity. M. zapota (shake flask method: 98.7%) and A . heterophyllus (shake flask method: 35.6%) seeds recorded the highest and lowest in vitro invertase inhibitory activity, respectively. The two‐dimensional thin‐layer chromatography and two‐dimensional preparative thin‐layer chromatography (2 D PTLC ) analyses have demonstrated the presence of polyphenols in M. zapota and A . heterophyllus seeds. The 2 D PTLC eluate of M. zapota has recorded a significant invertase inhibitory activity. Liquid chromatography‐mass spectrometry photo diode array analysis of M. zapota has exhibited the presence of four different polyphenols such as benzoyl hexosyl methyl luteolin sulfate ( m/z 662.1), 6 C /8 C hexosyl, 6 C /8 C pentosyl apigenin ( m/z 568), one unknown carboxylated flavonol glycoside ( m/z 484.7) and one unknown flavone derivative ( m/z 473.8). Practical Applications The fruits of M anilkara zapota and A rtocarpus heterophyllus have been consumed for their sweet delicious nature by most A sian community. Ethnobotanically, many parts of the above said species have been taken orally to treat various ailments. In the current scenario, appropriate food formulation as a health mix may be tried along with the seeds of M. zapota and/or A . heterophyllus to have a balanced glycemic control in the biological system.