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Evaluation of In Vitro Invertase Inhibitory Activity of M anilkara zapota Seeds – A Novel Strategy to Manage Diabetes Mellitus
Author(s) -
Sathishkumar Thiyagarajan,
Anitha Srinivasan,
Sharon Rajakumar Esther,
Santhi Velayudham,
Sukanya Mani,
Kumaraesan Kuppamuthu,
Rapheal Vinohar Stephen
Publication year - 2015
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12157
Subject(s) - artocarpus , syzygium , invertase , chemistry , mangifera , polyphenol , flavonoid , quercetin , column chromatography , traditional medicine , botany , sucrose , horticulture , food science , chromatography , biochemistry , biology , antioxidant , medicine
A rtocarpus heterophyllus (jack fruit), M anilkara zapota (sapota), M angifera indica (mango), V itis vinifera (grapes), C itrus sinensis (orange) and S yzygium cumini (jambul) seeds were selected to evaluate the in vitro invertase inhibitory activity. M. zapota (shake flask method: 98.7%) and A . heterophyllus (shake flask method: 35.6%) seeds recorded the highest and lowest in vitro invertase inhibitory activity, respectively. The two‐dimensional thin‐layer chromatography and two‐dimensional preparative thin‐layer chromatography (2 D PTLC ) analyses have demonstrated the presence of polyphenols in M. zapota and A . heterophyllus seeds. The 2 D PTLC eluate of M. zapota has recorded a significant invertase inhibitory activity. Liquid chromatography‐mass spectrometry photo diode array analysis of M. zapota has exhibited the presence of four different polyphenols such as benzoyl hexosyl methyl luteolin sulfate ( m/z 662.1), 6 C /8 C hexosyl, 6 C /8 C pentosyl apigenin ( m/z 568), one unknown carboxylated flavonol glycoside ( m/z 484.7) and one unknown flavone derivative ( m/z 473.8). Practical Applications The fruits of M anilkara zapota and A rtocarpus heterophyllus have been consumed for their sweet delicious nature by most A sian community. Ethnobotanically, many parts of the above said species have been taken orally to treat various ailments. In the current scenario, appropriate food formulation as a health mix may be tried along with the seeds of M. zapota and/or A . heterophyllus to have a balanced glycemic control in the biological system.