Anti‐Amyloidogenic Properties of D ryopteris Crassirhizoma Roots in A lzheimer's Disease Cellular Model

Abstract The inhibitory effects of D ryopteris crassirhizoma roots on A β production, which is one of the major causes of A lzheimer's disease, were investigated as they have not been studied at all. The methanol extract of D . crassirhizoma roots significantly reduced the production of A β in Chinese Hamster Ovarian cells overexpressing amyloid precursor proteins ( APPs ). This reduction was more pronounced with the butanol layer of D . crassirhizoma roots, which is partitioned based on solvent polarity. In addition, reduced production of A β by D . crassirhizoma roots was accompanied with increased production of sAPP β, which is a proteolytic fragment of APP by β‐secretases. The expression of β‐secretases was also decreased by treatment with D . crassirhizoma roots. Furthermore, the amount of CTFα , a proteolytic fragment of APP by α‐secretases, is significantly increased by D . crassirhizoma roots. These results indicate that D . crassirhizoma roots efficiently decreased A β production by inhibiting β‐secretases and increasing α‐secretases. Practical Applications D ryopteris crassirhizoma roots efficiently reduced A β production. Therefore, D . crassirhizoma has the potential to be developed into an anti‐ A β agent, which may have beneficial effects for the prevention or treatment of A lzheimer's disease.