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Inhibition of Bigeye Snapper ( P riacanthus Macracanthus ) Proteinases by Trypsin Inhibitor from Yellowfin Tuna ( T hunnus Albacores ) Roe
Author(s) -
Klomklao Sappasith,
Benjakul Soottawat,
Simpson Benjamin K.
Publication year - 2015
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12148
Subject(s) - yellowfin tuna , proteolysis , trypsin , autolysis (biology) , thunnus , chemistry , haddock , kunitz sti protease inhibitor , trypsin inhibitor , fishery , biology , food science , biochemistry , tuna , enzyme , fish <actinopterygii>
The inhibitory effect of partially purified trypsin inhibitor from yellowfin tuna ( T hunnus albacores ) roe ( TIYTR ) on proteolysis of bigeye snapper ( P riacanthus macracanthus ) proteinases was investigated. TIYTR inhibited sarcoplasmic proteinases and autolysis of bigeye snapper mince and its washed mince at 60C in a concentration‐dependent manner. Myosin heavy chain ( MHC ) in the mince and the washed mince of bigeye snapper was better retained when higher concentrations of TIYTR were used. The presence of NaCl (3.0% w/w) slightly enhanced the inhibitory activity of TIYTR (3.5–5.8%). Both TIYTR and beef plasma protein (at a level of incorporation of 3% w/w) showed higher inhibition of bigeye snapper proteinases than egg white ( P < 0.05). Based on inhibition studies, it is suggested that the trypsin inhibitor from the roe of yellowfin tuna can be a potential aid to suppress the gel weakening of bigeye snapper surimi, elicited by trypsin‐like proteinases, during either setting or heating. Practical Applications Yellowfin tuna ( T hunnus albacores ) roe is an abundant and underutilized resource that can be used as a source of trypsin inhibitors. Trypsin inhibitors from the roe of yellowfin tuna play a role in the inhibition of proteolysis in bigeye snapper ( P riacanthus macracanthus ) muscle. Thus, it can be a potential alternative for commercial trypsin inhibitor to inhibit proteolysis and enhance surimi gel strength.