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Phenolic Profile, Antioxidant Activities and Genotoxic Evaluations of C alendula officinalis
Author(s) -
Mubashar Sabir Syed,
Khan Muhammad Fareed,
Rocha Joao Batista Texeira,
Boligon Aline Augusti,
Athayde Margareth Linde
Publication year - 2015
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12132
Subject(s) - calendula officinalis , antioxidant , chemistry , officinalis , oxidative stress , flavonoid , dpph , traditional medicine , lipid peroxidation , pharmacology , biochemistry , medicine
Our study aims to evaluate the antioxidant and genotoxic activities obtained from hot water extracts of flowers and leaves of C alendula officinalis L inn. (Compositae). The extracts effectively prevented the lipid peroxidation induced by different prooxidants (10 μM FeSO4 and 5 μM sodium nitroprusside) in rat liver and brain homogenates. Moreover, the free radical scavenging activities of the extracts were evaluated by the quenching of 2,2‐diphenyl‐1‐picrylhydrazyl ( IC 50 = 184.16 μg/mL) and deoxyribose ( IC 50 = 28.1 μg/mL) assays. The results obtained on alkaline comet assay revealed that exposure of human lymphocytes to aqueous extract of flower at a relatively high concentration (0.4 mg/mL) did not induce genotoxic effects. The major phenolic acids, some flavonoid aglycone and glycosides, were identified in flower and leaves by high performance liquid chromatography. These results indicate that C . officinalis has a significant antioxidant activity and can be effectively utilized against oxidative stress‐related diseases. Practical Applications C . officinalis is a medicinal plant which has been used in traditional medicine for skin complaints, wounds and burns, conjunctivitis, poor eyesight, menstrual irregularities, varicose veins, hemorrhoids and duodenal ulcers. As oxidative stress has been implicated as one of the inducing factors for the development of various metabolic disorders, the present study proposes to evaluate the antioxidant potential of flower and leaf extracts of plant, which may prove to be beneficial against these disorders.