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Hepatoprotective Effect of L entinus edodes Mycelia Fermented Formulation against Alcoholic Liver Injury in Rats
Author(s) -
Chung WonSeok,
Wang JingHua,
Bose Shambhunath,
Park JongMin,
Park SunOk,
Lee SangJong,
Jeon Songhee,
Kim Hojun
Publication year - 2015
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12124
Subject(s) - chemistry , alcoholic liver disease , liver injury , malondialdehyde , pharmacology , lactulose , biochemistry , medicine , oxidative stress , cirrhosis
Abstract The hepatoprotective effects of fermented black rice bran extracts ( FF 1 and FF 2: black rice bran fermented by L entinus edodes derived from mycelium supplemented with soybean or H ovenia dulcis ) and their associated mechanisms were evaluated. In an in vitro experiment, FFs caused significant amelioration of the metabolic function of rat hepatocytes treated with NH 4 Cl . In addition, administration of FFs to rats with chronic liver injury induced by 12‐week continual alcohol consumption resulted in significant restoration of body weight shrinkage, notable attenuation of excessive aspartate aminotransferase, alkaline phosphatase and endotoxin in serum, malondialdehyde in liver and the lactulose/mannitol ratio in urine. Furthermore, FF 1 or FF 2 also caused significant downregulation of gene expression of several critical inflammatory mediators (interleukin‐6, tumor necrosis factor‐alpha, cyclooxygenase‐2 and inducible nitric oxide synthase). Histopathological findings also indicated that FFs reduced inflammation, necrosis and fatty infiltration in liver tissue. Taken together, FFs exert hepatoprotective effects through anti‐inflammatory and anti‐lipid peroxidative properties and regulation of intestinal permeability. Practical Applications In this study, mycelia fermentation was utilized as a feasible strategy for enhancing the hepatotherapeutic effect of black rice and herbs. The findings demonstrated that H ovenia dulcis exerts a synergistic protective effect on an alcoholic liver disease animal model and could provide a new effective potential approach to alleviate alcoholic liver diseases.