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Edible Brown Alga E cklonia cava Derived Phlorotannin‐Induced Anti‐Adipogenic Activity in Vitro
Author(s) -
Kong ChangSuk,
Kim Haejin,
Seo Youngwan
Publication year - 2015
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12093
Subject(s) - adipogenesis , fatty acid synthase , biochemistry , peroxisome , fatty acid , perilipin , fatty acid binding protein , adipocyte protein 2 , biology , chemistry , adipose tissue , receptor , adipocyte , gene
The present study performed the bioactivity‐monitored isolation of anti‐adipogenic phlorotannins from E cklonia cava as a part of the ongoing research to develop antiobesity nutraceuticals from natural origin. Extracts were partitioned into n ‐hexane, 85% aqueous MeOH , n ‐ BuOH and water fractions. Their adipogenic activities were examined by measuring glycerol release level and adipogenic‐related gene expression in differentiating 3 T 3‐ L 1 preadipocytes. Among them, n ‐ BuOH fractions significantly increased glycerol secretion and reduced the regulation of adipogenic transcription factors, peroxisome proliferator‐activated receptor‐γ ( PPAR γ) and CCAAT /enhancer‐binding protein α ( C/EBP α), as well as tumor necrosis factor α ( TNF α). Further separation from n ‐ BuOH fraction led to the isolation of phlorotannins, triphlorethol‐ A , eckol and dieckol. Phlorotannins increased the glycerol secretion and reduced the glucose consumption levels of 3 T 3‐ L 1 adipocytes. Moreover, the phlorotannins reduced the expression levels of PPAR γ, C / EBP α and differentiation‐dependent factor 1/sterol regulatory element‐binding protein 1c, as well as downstream genes such as fatty acid binding protein‐4, fatty acid transport protein‐1, fatty acid synthase, L eptin and acyl‐ CoA synthetase 1. In addition, phlorotannins increased the mRNA expression of hormone‐sensitive lipase while suppressing perilipin and TNF α expressions. Practical Applications Brown alga, E cklonia cava , is one of the most common edible algae in E ast A sian countries and widely consumed throughout K orea and J apan because of its nutritional importance along with its medicinal value. Constituents of E . cava , especially phenolic compounds, are considered to possess several bioactivities against various complications and diseases. In this regard, this study provides valuable insights concerning the antiobesity potential of E . cava and effective ingredients. Results indicate that the isolated phlorotannins act as antiobesity agents and suggest possible mechanisms of action. Following this study, a better utilization of E . cava via proper extraction of antiobesity constituents will pave the way for future nutraceuticals with antiobesity value.