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Treatment with C aulerpa Microphysa Pepsin‐Digested Extract Induces Apoptosis in Murine Leukemia WEHI ‐3 Cells
Author(s) -
Chou SuTze,
Lin HuiChiu,
Chuang MeiYu,
Chiu TsaiHsin
Publication year - 2014
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.12079
Subject(s) - apoptosis , reactive oxygen species , flow cytometry , cyclin dependent kinase 6 , chemistry , pepsin , cyclin d1 , cyclin dependent kinase , caspase , caspase 3 , cell cycle checkpoint , microbiology and biotechnology , cell cycle , pharmacology , biology , biochemistry , programmed cell death , enzyme
Abstract In this study, we examined the possible apoptotic mechanism of pepsin‐digested extract of C aulerpa microphysa ( CME ) in myelomonocytic leukemia ( WEHI ‐3) cells. Flow cytometry demonstrated that CME induced cell cycle arrest in the G 0/ G 1 phase and stimulated reactive oxygen species ( ROS ) production and calcium release but caused a loss of the mitochondrial membrane potential ( MMP ). The results indicated that the protein levels of cyclin D, cyclin E, CDK 6, CDK 2 and Bcl ‐2 decreased and those of p 21, p 27, p 53, Bax , Bid , GRP 78, GADD 153, apoptosis‐inducing factor ( AIF ), caspase‐3 and caspase‐9 increased in WEHI ‐3 cells after CME treatment. In conclusion, CME induced G 0/ G 1 phase arrest, decreased MMP and increased Ca 2+ release and ROS production in the WEHI ‐3 cells. The results suggest that CME may have potential as an anticancer agent. Practical Applications C aulerpa microphysa ( CME ) has multiple functions and has been applied as natural seaweed extracts in the food and pharmaceutical industries. This study revealed for the first time that CME treatments inhibited the expression of the anti‐apoptotic protein and promoted the expression of pro‐apoptotic proteins. The results suggest that CME could be functional in food and pharmaceutical industries and could be an alternative anticancer medicine in the future.