Effect of anticoagulants on farmed giant kokopu,   Galaxias argenteus   (Gmelin 1789) haematological parameters and erythrocyte fragility | Zendy

Lulijwa Ronald | Zendy; Alfaro Andrea C. | Zendy; Young Tim | Zendy; Venter Leonie | Zendy; Decker Paul | Zendy; Merien Fabrice | Zendy; Meyer Jill | Zendy

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Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility

Author(s) -

Lulijwa Ronald,

Alfaro Andrea C.,

Young Tim,

Venter Leonie,

Decker Paul,

Merien Fabrice,

Meyer Jill

Publication year - 2021

Publication title -

journal of fish biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.672

H-Index - 115

eISSN - 1095-8649

pISSN - 0022-1112

DOI - 10.1111/jfb.14746

Subject(s) - biology , haemolysis , anticoagulant , ethylenediaminetetraacetic acid , medicine , immunology , chelation , chemistry , organic chemistry

We sought to determine a compatible anticoagulant for routine haematological and physiological assessments with giant kokopu ( Galaxias argenteus ), an endemic New Zealand fish. We observed that blood treated with lithium heparin (LH) rapidly coagulated and haemolysed, making it unsuitable for G. argenteus . Dipotassium ethylenediaminetetraacetic acid (K 2 EDTA) and trisodium citrate (citrate) effectively prevented blood coagulation. K 2 EDTA‐treated erythrocytes exhibited the least mean haemolysis and mean corpuscular fragility. Further studies into prolonged storage effects of citrate and K 2 EDTA are recommended to find a compatible anticoagulant for use with G. argenteus blood.

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