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Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility
Author(s) -
Lulijwa Ronald,
Alfaro Andrea C.,
Young Tim,
Venter Leonie,
Decker Paul,
Merien Fabrice,
Meyer Jill
Publication year - 2021
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.14746
Subject(s) - biology , haemolysis , anticoagulant , ethylenediaminetetraacetic acid , medicine , immunology , chelation , chemistry , organic chemistry
We sought to determine a compatible anticoagulant for routine haematological and physiological assessments with giant kokopu ( Galaxias argenteus ), an endemic New Zealand fish. We observed that blood treated with lithium heparin (LH) rapidly coagulated and haemolysed, making it unsuitable for G. argenteus . Dipotassium ethylenediaminetetraacetic acid (K 2 EDTA) and trisodium citrate (citrate) effectively prevented blood coagulation. K 2 EDTA‐treated erythrocytes exhibited the least mean haemolysis and mean corpuscular fragility. Further studies into prolonged storage effects of citrate and K 2 EDTA are recommended to find a compatible anticoagulant for use with G. argenteus blood.