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Molecular cloning and expression analysis of myd88 from oriental weatherfish ( Misgurnus anguillicaudatus ) in response to bacterial challenge
Author(s) -
Chen Jian H.,
Li Xue,
Wang Meng J.,
Wang Chun M.,
Peng Yan Q.,
Wang Hai H.,
Zhu Ming
Publication year - 2020
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.14289
Subject(s) - biology , misgurnus , complementary dna , open reading frame , microbiology and biotechnology , messenger rna , innate immune system , immune system , rapid amplification of cdna ends , blot , gene , molecular cloning , genetics , peptide sequence , fishery , fish <actinopterygii>
Myeloid differentiation factor 88 (Myd88) plays an important role in both innate and adaptive immune response. In this study, the full‐length complementary DNA (cDNA) of myd88 from Misgurnus anguillicaudatus was characterized. The myd88 cDNA is 1333 bp in length and contains an 855 bp open reading frame encoding a predicted protein of 284 amino acids. The predicted protein possesses typical Myd88 domain structural features including a death domain in the N‐terminus, and box 1, 2, and 3 motifs of the Toll/IL‐1 receptor domain in the C‐terminus. Quantitative real‐time PCR (qRT‐PCR) revealed that myd88 messenger RNA (mRNA) was ubiquitously expressed in all examined tissues, especially highly in brain, kidney, blood, intestines and liver. qRT‐PCR and western blotting were used to determine the mRNA and protein levels of Myd88 after Aeromonas veronii challenge, respectively. The Myd88 was remarkably upregulated in response to infection of A. veronii. These results suggested that Myd88 may play a vital role during the immune response of M. anguillicaudatus against bacterial infection.