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Virus susceptibility of a new cell line derived from the fin of black carp Mylopharyngodon piceus
Author(s) -
Wang Yizhou,
Xue Ting,
Wang Qian,
Xia Bilin,
Pan Qihua,
Chen Tiansheng
Publication year - 2020
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.14215
Subject(s) - biology , common carp , grass carp , microbiology and biotechnology , fishery , fish <actinopterygii> , cyprinus
A continuous cell line MPF derived from the fin of black carp Mylopharyngodon piceus was established and characterised in this study. Mylopharyngodon piceus fin (MPF) cells were subcultured for more than 80 passages with high viability recovery after long‐term storage. The karyotyping analysis revealed that MPF had a modal diploid chromosome number (2 n = 48) and identical ribosomal RNA sequence with black carp. In addition, the expression of pluripotency‐associated markers including nanog , oct4 and vasa , were detected in MPF. The transient transfection efficiency of MPF reached 23% with a fluorescent reporter by modified electroporation and stable expression of red fluorescent MPF was established by the baculovirus system, indicating that MPF is an ideal platform for studying gene functions in vitro . Lastly, cytopathic effects were also observed and RNA transcripts of a viral gene increased after infection by spring viremia of carp virus (SVCV), suggesting that MPF could be an alternative tool for investigating pathogen‐host interactions in black carp. In conclusion, a fin cell line that is susceptible to SVCV was established as a potential adult stem‐cell line, providing a suitable tool for future genetic analyses and pathogen–host studies in black carp.