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Effects of temperature on sperm motility of burbot Lota lota : spontaneous activation and calcium dependency
Author(s) -
Dadras Hadiseh,
Boryshpolets Sergey,
Golpour Amin,
Policar Tomas,
Blecha Miroslav,
Dzyuba Borys
Publication year - 2019
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.14110
Subject(s) - spermatozoon , motility , biology , calcium , sperm motility , sperm , andrology , human fertilization , anatomy , medicine , microbiology and biotechnology , botany
Several factors regulating activation of spermatozoon motility in Eurasian burbot, Lota lota , including osmolality, calcium (Ca 2+ ) ions, and temperature were investigated. Spermatozoon motility in Eurasian burbot, Lota lota was assessed at 4 and 30°C in seminal fluid, isotonic media (with and without Ca 2+ ) and hypotonic media (with and without Ca 2+ ). Spermatozoa were spontaneously activated in seminal fluid at 20°C and the maximum motility was recorded at 30°C, which is out of the spawning temperature range, indicating that no risk of activation occurs during routine semen handling in artificial insemination. Initiation of spermatozoon motility in L. lota is mediated by Ca 2+ and sensitivity to Ca 2+ is dependent on temperature.