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Use of mucus as a non‐invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)
Author(s) -
Domingues Rodrigo R.,
GarroneNeto Domingos,
Hilsdorf Alexandre W. S.,
Gadig Otto B. F.
Publication year - 2019
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13919
Subject(s) - biology , dna barcoding , mucus , zoology , sampling (signal processing) , fishery , anatomy , ecology , computer science , filter (signal processing) , computer vision
In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99–100% accuracy to the species level. This method proved to provide reliable and good‐quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.