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Differential expression of HPG‐axis genes in autotetraploids derived from red crucian carp Carassius auratus red var., ♀ × blunt snout bream Megalobrama amblycephala , ♂
Author(s) -
Qin Qin B.,
Liu Qi W.,
Zhou Yu W.,
Wang Chong Q.,
Qin Huan,
Zhao Chun,
Liu Shao J.
Publication year - 2018
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13818
Subject(s) - megalobrama , biology , crucian carp , carassius auratus , gene , grass carp , genetics , sexual maturity , gene expression , fish <actinopterygii> , fishery , endocrinology
Autotetraploid fish (4 n = 200, abbreviated as 4 n RR), which reach sexual maturity at 1 year of age, were derived from the whole genome duplication of red crucian carp Carassius auratus red var. (RCC; 2 n = 100) and possess four sets of chromosomes from RCC. The histological features of the gonads showed that the RCC and 4 n RR both possessed normal gonadal structure and could arrive at maturation. To understand the expression characteristics of genes related to reproductive development in the autotetraploid fish, we analysed the nucleotide sequence and expression characteristics of the gnrh 2, gthb and gthr genes, which are the pivotal genes of the hypothalamic–pituitary–gonadal (HPG) axis. We found that the gnrh 2, gthb and gthr genes in 4 n RR share remarkable homology with RCC, but there were obvious differences in expression levels between 4 n RR and RCC. These results demonstrate that autotetraploidization can lead to gene expression changes. This study provides insights into the molecular mechanism underlying the reproductive development of autotetraploid fish and is expected to be of great significance for subsequent research on polyploidization.