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Comparison of vegetable shortening and cocoa butter as vehicles for cortisol manipulation in Salmo trutta
Author(s) -
BirnieGauvin K.,
Peiman K. S.,
Larsen M. H.,
Aarestrup K.,
Gilmour K. M.,
Cooke S. J.
Publication year - 2018
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13513
Subject(s) - salmo , biology , temperate climate , brown trout , zoology , hydrocortisone , food science , endocrinology , fishery , fish <actinopterygii> , ecology
This study demonstrates that vegetable shortening and cocoa butter are two effective vehicles for intraperitoneal cortisol implants in juvenile teleosts, specifically brown trout Salmo trutta , residing in north temperate freshwater environments. Each vehicle showed a different pattern of cortisol elevation. Vegetable shortening was found to be a more suitable vehicle for long‐term cortisol elevation [elevated at 3, 6 and 9 days post treatment (dpt)], while cocoa butter may be better suited for short‐term cortisol elevation (only elevated at 3 dpt). Additionally, plasma cortisol levels were higher with cortisol–vegetable shortening than with cortisol–cocoa butter implants. Plasma glucose levels were elevated 6 and 9 dpt for fishes injected with cortisol–vegetable shortening, but did not change relative to controls and shams in cortisol–cocoa butter fishes. In conclusion, vegetable shortening and cocoa butter are both viable techniques for cortisol manipulation in fishes in temperate climates, providing researchers with different options depending on study objectives.