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Di‐2‐ethylhexyl phthalate (DEHP) exposure disturbs lipid metabolism in juvenile yellow catfish Tachysurus fulvidraco
Author(s) -
Meng F. X.,
Li M.,
Song M. Z.,
Yuan L. X.,
Gong Y. F.,
Qian Y. X.,
Shi G.,
Wang R. X.
Publication year - 2018
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13493
Subject(s) - catfish , phthalate , biology , medicine , endocrinology , lipid metabolism , lipoprotein lipase , metabolism , peroxisome , fatty acid synthase , carnitine , biochemistry , chemistry , fish <actinopterygii> , receptor , adipose tissue , fishery , organic chemistry
This study was conducted to determine the mechanism by which di‐2‐ethylhexyl phthalate (DEHP) exposure influences lipid metabolism of juvenile yellow catfish Tachysurus fulvidraco . Fish were exposed to three DEHP concentrations (0, 0·1 and 0·5 mg l −1 DEHP) for 8 weeks. Fatty acid synthase (FAS) activity significantly decreased with increasing DEHP concentrations, the highest value was in the Tween control group, whereas the lowest activities of carnitine palmitoyltransferase (CPT) and lipoprotein lipase (LPL) were in this group. The messenger (m)RNA levels of 6‐phospho‐gluconate dehydrogenase (6PGD), FAS and acetyl‐CoA carboxylase a (ACCa) significantly increased with increasing DEHP concentration, the highest values were in the 0·5 mg l −1 DEHP group. The mRNA level of peroxisome proliferator‐activated receptor γ (PPARγ) was lower in Tween control than in fish exposed to 0·1 and 0·5 mg l −1 DEHP. The highest mRNA level of ACCb was in the 0·1 mg l −1 DEHP group. These results indicate that DEHP exposure can disturb lipid metabolism at the enzymatic and mRNA levels in Pelteobagrus fulvidraco .