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Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli
Author(s) -
Zhang W.,
Tang X. Q.,
Sheng X. Z.,
Xing J.,
Zhan W. B.
Publication year - 2017
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13279
Subject(s) - sebastes , edwardsiella tarda , biology , monoclonal antibody , antibody , microbiology and biotechnology , immunofluorescence , scorpaenidae , flow cytometry , immunoglobulin m , immunology , immunoglobulin g , fishery , fish <actinopterygii>
Monoclonal antibodies ( mAbs ) against black rockfish Sebastes schlegeli serum immunoglobulin M ( IgM ) were developed, which showed a specific reaction with the heavy chain of S. schlegeli IgM in Western blotting and with surface IgM positive ( sIgM + ) lymphocytes in indirect immunofluorescence. mAb 2A6 was employed to investigate the antibody and sIgM + lymphocyte responses of S. schlegeli injected with inactivated Edwardsiella tarda , by ELISA and flow cytometry. Compared with controls, the level of specific antibodies and the percentage of sIgM + lymphocytes both increased in the immunized fish and simultaneously reached their peaks at day 35 after immunization.