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Effects of storage time on the motility, mortality and calcium levels of Atlantic salmon Salmo salar spermatozoa
Author(s) -
Parodi J.,
Guerra G.,
Cuevas M.,
RamírezReveco A.,
Romero F.
Publication year - 2017
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.13245
Subject(s) - milt , salmo , biology , motility , calcium , calcium in biology , andrology , zoology , fishery , intracellular , medicine , fish <actinopterygii> , biochemistry , microbiology and biotechnology
This study estimates spermatozoa mortality, morphology, motility and intracellular calcium levels in Atlantic salmon Salmo salar milt after prolonged storage. Milt samples were preserved at 4° C for 25 days and then evaluated for mortality. Motility remained high for the first 3 days and the mortality was low during the first 5 days of storage. A decrease of >50% in calcium content was observed after 5 days of storage. When spermatozoa were activated, calcium levels increased >200% in relative fluorescence units ( RFU ); this rate of increase was lost when the samples were stored for extended periods of time and was only partially manifested in a zero calcium solution. The results suggest that in vitro storage of S. salar spermatozoa at 4° C for a period of 3 days preserves motility and limits mortality to levels similar to those of fresh spermatozoa. This method also maintains intracellular calcium storage critical for spermatozoa performance.