Real‐time  PCR  identification of lake whitefish  Coregonus clupeaformis  in the  L aurentian  G reat  L akes | Zendy

Overdyk L. M. | Zendy; Braid H. E. | Zendy; Naaum A. M. | Zendy; Crawford S. S. | Zendy; Hanner R. H. | Zendy

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Real‐time PCR identification of lake whitefish Coregonus clupeaformis in the L aurentian G reat L akes

Author(s) -

Overdyk L. M.,

Braid H. E.,

Naaum A. M.,

Crawford S. S.,

Hanner R. H.

Publication year - 2016

Publication title -

journal of fish biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.672

H-Index - 115

eISSN - 1095-8649

pISSN - 0022-1112

DOI - 10.1111/jfb.12922

Subject(s) - coregonus clupeaformis , amplicon , biology , standard curve , real time polymerase chain reaction , fish <actinopterygii> , microbiology and biotechnology , mitochondrial dna , polymerase chain reaction , fishery , gene , genetics , biochemistry

The purpose of this study was to develop a real‐time PCR assay to specifically identify lake whitefish Coregonus clupeaformis in larval fish assemblages based on a 122 bp amplicon from the mitochondrial genome. The efficiency of the reaction, as calculated from the standard curve, was 90·77% with the standard curve having an r 2 value of 0·998. Specificity of the assay provided single melt peak in a melt‐curve analysis and amplification of only the target species. The assay successfully identified target DNA in as low as 0·1% proportion of a DNA mixture. This assay was designed on the portable Smart Cycler II platform and can be used in both field and laboratory settings to successfully identify C. clupeaformis .

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