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Isolation and characterization of 13 polymorphic microsatellite loci in Hypostomus ancistroides (Teleostei, Loricariidae) and cross‐amplification in related species
Author(s) -
Galindo B. A.,
Ferreira D. G.,
Almeida F. S.,
Carlsson J.,
Sofia S. H.
Publication year - 2015
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12675
Subject(s) - loricariidae , biology , teleostei , isolation (microbiology) , evolutionary biology , microsatellite , zoology , genetics , fishery , fish <actinopterygii> , gene , bioinformatics , allele , catfish
This study details 13 novel polymorphic microsatellite loci in the armoured catfish Hypostomus ancistroides , and assesses their utility for population genetic studies. The analysis of 30 individuals revealed a total of 99 different alleles (ranging from two to 15 alleles per locus), with an average of 7·62 alleles per locus, with observed and expected heterozygosities ranging from 0·103 to 0·931 and from 0·102 to 0·906, respectively. One of the 13 loci showed significant deviations from Hardy–Weinberg equilibrium, probably due to the presence of null alleles, inferred from the excess of homozygotes.