Isolation and characterization of tri‐ and tetra‐repeat microsatellite loci in the white‐spotted charr  Salvelinus leucomaenis  (Salmonidae) | Zendy

Yamamoto S. | Zendy; Sekino M. | Zendy

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Isolation and characterization of tri‐ and tetra‐repeat microsatellite loci in the white‐spotted charr Salvelinus leucomaenis (Salmonidae)

Author(s) -

Yamamoto S.,

Sekino M.

Publication year - 2015

Publication title -

journal of fish biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.672

H-Index - 115

eISSN - 1095-8649

pISSN - 0022-1112

DOI - 10.1111/jfb.12628

Subject(s) - biology , microsatellite , genetics , white (mutation) , allele , salvelinus , evolutionary biology , pyrosequencing , zoology , salmonidae , rainbow trout , fishery , trout , gene , fish <actinopterygii>

Tri‐ and tetra‐motif repeat microsatellite marker loci were developed for the white‐spotted charr Salvelinus leucomaenis . The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite‐primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180–0·600 and 0·188–0·599, respectively.

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