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Characterizations of four toll‐like receptor 4s in grass carp Ctenopharyngodon idellus and their response to grass carp reovirus infection and lipopolysaccharide stimulation
Author(s) -
Pei Y. Y.,
Huang R.,
Li Y. M.,
Liao L. J.,
Zhu Z. Y.,
Wang Y. P.
Publication year - 2015
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12617
Subject(s) - grass carp , biology , lipopolysaccharide , tlr4 , downregulation and upregulation , stimulation , toll like receptor , subcellular localization , receptor , gene , microbiology and biotechnology , fishery , immunology , endocrinology , signal transduction , innate immune system , cytoplasm , biochemistry , fish <actinopterygii>
In this study, the subcellular localization, tissue distribution and response to grass carp reovirus ( GCRV ) infection and lipopolysaccharide ( LPS ) stimulation of four grass carp Ctenopharyngodon idellus toll‐like receptor 4 ( tlr4 ) genes were investigated. All four genes were constitutively expressed in all tissues studied, but the subcellular localization and tissue exhibiting the highest expression differed for each protein. Following GCRV infection, all the four tlr4s were upregulated in all tissues examined, and stimulation of C. idellus kidney ( CIK ) cells with LPS resulted in downregulation of all four tlr4s . These results provide a foundation for further investigation of tlr4 genes in bony fishes.

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