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Isolation and characterization of 16 polymorphic microsatellite loci for Euchiloglanis kishinouyei
Author(s) -
Li Y.,
Wang J.,
Zhao K.,
Xie B.,
Peng Z.
Publication year - 2014
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12472
Subject(s) - biology , microsatellite , genetics , allele , population , allele frequency , genomic library , evolutionary biology , gene , base sequence , demography , sociology
A total of 16 novel polymorphic microsatellite marker loci were isolated from a genomic library of Euchiloglanis kishinouyei and further characterized using a sample from a wild population consisting of 40 individuals. The number of alleles among loci ranged from three to 17 and the observed and expected heterozygosities ranged from 0·030 to 0·950 and 0·163 to 0·980, respectively. The average polymorphic information content ( PIC ) of all loci was 0·450.