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Molecular characterization and expression of cyp19a gene in Carassius auratus
Author(s) -
Chen X. W.,
Jiang S.,
Gu Y. F.,
Shi Z. Y.
Publication year - 2014
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12418
Subject(s) - biology , exon , steroidogenic factor 1 , intron , gene , carassius auratus , hatching , promoter , aromatase , microbiology and biotechnology , genetics , gene expression , ovary , fish <actinopterygii> , transcription factor , fishery , ecology , nuclear receptor , cancer , breast cancer
A cyp19a gene that contains nine exons and eight introns was identified from Carassius auratus and was mainly expressed in the ovary. The cyp19a mRNA level after hatching was initially low, but began to increase from 25 days after hatching. A number of cis‐acting elements, such as the oestrogen receptor, steroidogenic factor 1 and SOX ‐5 recognition sites, were found in the promoter of the cyp19 gene, which possesses a promoter function confirmed by a recombination green fluorescent protein checking system in vitro .