A rapid  qPCR  method for genetic sex identification of  Salmo salar  and  Salmo trutta  including simultaneous elucidation of interspecies hybrid paternity by high‐resolution melt analysis | Zendy

Anglès d'Auriac M. B. | Zendy; Urke H. A. | Zendy; Kristensen T. | Zendy

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A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high‐resolution melt analysis

Author(s) -

Anglès d'Auriac M. B.,

Urke H. A.,

Kristensen T.

Publication year - 2014

Publication title -

journal of fish biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.672

H-Index - 115

eISSN - 1095-8649

pISSN - 0022-1112

DOI - 10.1111/jfb.12401

Subject(s) - salmo , biology , polymerase chain reaction , identification (biology) , aquaculture , zoology , fishery , genetics , gene , fish <actinopterygii> , ecology

This study presents an improved duplex quantitative polymerase chain reaction ( qPCR ) method using the master sex‐determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.

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