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A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high‐resolution melt analysis
Author(s) -
Anglès d'Auriac M. B.,
Urke H. A.,
Kristensen T.
Publication year - 2014
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12401
Subject(s) - salmo , biology , polymerase chain reaction , identification (biology) , aquaculture , zoology , fishery , genetics , gene , fish <actinopterygii> , ecology
This study presents an improved duplex quantitative polymerase chain reaction ( qPCR ) method using the master sex‐determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.