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Validation of otolith daily increments in early juveniles of shanny Lipophrys pholis
Author(s) -
Carvalho M. G.,
Moreira A. S.,
Moreira C.,
Queiroga H.,
Santos P. T.,
Correia A. T.
Publication year - 2014
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12333
Subject(s) - otolith , biology , alizarin red , tetracycline hydrochloride , fish <actinopterygii> , fishery , tetracycline , staining , genetics , antibiotics , microbiology and biotechnology
To assess the periodicity of micro‐increment formation in otoliths of Lipophrys pholis , 90 early juveniles were immersed in alizarin red S or tetracycline hydrochloride for 24 h and sacrificed after 10, 20 and 30 days. The number of micro‐increments viewed under light microscopy was significantly related to the duration of the experimental period, and the slopes of the linear regressions were not significantly different from 1. This study indicates that micro‐increments in sagittae were deposited daily and can be used as reliable sources of age information for L. pholis .