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In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata
Author(s) -
KalamarzKubiak H.,
MeiriAshkenazi I.,
Kleszczyńska A.,
Rosenfeld H.
Publication year - 2014
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12297
Subject(s) - medicine , biology , endocrinology , vasotocin , secretion , neuropeptide , in vitro , urotensin ii , arginine , receptor , biochemistry , amino acid
This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin ( AVT ) and isotocin ( IT ), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin ( UI ). Pituitary cells were exposed to 1·4 × 10 −8 , 1·4 × 10 −7 and 0·4 × 10 −6 M cortisol and 10 −12 , 10 −10 and 10 −8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography ( HPLC ). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI . It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal ( HPI ) axis in this species.