Premium
Characterization of a novel cell line from the caudal fin of koi carp Cyprinus carpio
Author(s) -
Lin S.L.,
Cheng Y.H.,
Wen C.M.,
Chen S.N.
Publication year - 2013
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12116
Subject(s) - biology , carp , cyprinus , immunocytochemistry , microbiology and biotechnology , cell culture , grouper , antibody , common carp , virus , fish <actinopterygii> , virology , immunology , fishery , endocrinology , genetics
A continuous cell line ( KF ‐101) derived from the caudal fin of the koi carp Cyprinus carpio was established and characterized. The KF ‐101 cell line multiplied abundantly in Leibovitz's L‐15 medium containing 10% foetal bovine serum at 25° C, and was subcultured for >90 passages over a period of 3 years. Immunocytochemistry revealed that the KF ‐101 cells contain keratin, junction proteins connexin‐43 and occludin, and ectodermal stem‐cell marker Pax‐6, but not vimentin. Furthermore, the KF ‐101 cells reacted with anti‐human DARPP ‐32 and anti‐human GATA ‐4 antibodies, and the labelling was regulated according to the cell cycle. The labels of the DARPP ‐32 and GATA ‐4 antibodies in the KF ‐101 cells were the suggested phosphatase‐1 inhibitor‐1 and GATA ‐3, respectively. In addition, the KF ‐101 cells were susceptible to koi herpesvirus but were resistant to eel herpesvirus, iridovirus, grouper nodavirus and chum salmon ( Oncorhynchus keta ) virus. The results indicate that the KF ‐101 cells are suitable materials for investigating biological and virological development.