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Neuropeptide Y in black seabream Acanthopagrus schlegelii : identification, distribution and mRNA expression responses to ghrelin
Author(s) -
Ma X. L.,
Zheng L. W.,
Mao L. T.,
Zhou L. B.,
Wang A. L.
Publication year - 2013
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12084
Subject(s) - biology , neuropeptide y receptor , ghrelin , messenger rna , hypothalamus , medicine , endocrinology , gene expression , coding region , reverse transcriptase , gene , rna , neuropeptide , hormone , receptor , genetics
The coding region of neuropeptide Y (NPY) complementary (c)DNA was cloned from the hypothalamus RNA of black seabream Acanthopagrus schlegelii , including 297 bp coding for prepro‐NPY of 98 amino acids. Real‐time reverse transcriptase‐polymerase chain reaction was performed to determine A. schlegelii npy gene expression; NPY messenger RNA (mRNA) was expressed highly in the brain and stomach. Treatment with acylated ghrelin significantly up‐regulated NPY mRNA level in the hypothalamus, suggesting that NPY may be involved in regulating food intake of A. schlegelii .