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Assessment of DNA damage in sperm after repeated non‐invasive sampling in zebrafish Danio rerio
Author(s) -
Reinardy H. C.,
Skippins E.,
Henry T. B.,
Jha A. N.
Publication year - 2013
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/jfb.12042
Subject(s) - sperm , biology , danio , comet assay , andrology , dna damage , zebrafish , dna , microbiology and biotechnology , anatomy , genetics , gene , medicine
Repeated non‐invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single‐cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide ( H 2 O 2 , 0–200 µM ), and in vitro exposure of sperm to 200 µM H 2 O 2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e ., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.