Assessment of  DNA  damage in sperm after repeated non‐invasive sampling in zebrafish  Danio rerio | Zendy

Reinardy H. C. | Zendy; Skippins E. | Zendy; Henry T. B. | Zendy; Jha A. N. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Assessment of DNA damage in sperm after repeated non‐invasive sampling in zebrafish Danio rerio

Author(s) -

Reinardy H. C.,

Skippins E.,

Henry T. B.,

Jha A. N.

Publication year - 2013

Publication title -

journal of fish biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.672

H-Index - 115

eISSN - 1095-8649

pISSN - 0022-1112

DOI - 10.1111/jfb.12042

Subject(s) - sperm , biology , danio , comet assay , andrology , dna damage , zebrafish , dna , microbiology and biotechnology , anatomy , genetics , gene , medicine

Repeated non‐invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single‐cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide ( H 2 O 2 , 0–200 µM ), and in vitro exposure of sperm to 200 µM H 2 O 2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e ., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research