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mRNA Polyadenylation Machineries in Intestinal Protozoan Parasites
Author(s) -
OspinaVilla Juan David,
TovarAyona Brisna Joana,
LópezCamarillo César,
SotoSánchez Jacqueline,
RamírezMoreno Esther,
CastañónSánchez Carlos A.,
Marchat Laurence A.
Publication year - 2020
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12781
Subject(s) - polyadenylation , biology , giardia lamblia , entamoeba histolytica , entamoeba , cryptosporidium parvum , genetics , gene , giardia , rna
In humans, mRNA polyadenylation involves the participation of about 20 factors in four main complexes that recognize specific RNA sequences. Notably, CFIm25, CPSF73, and PAP have essential roles for poly(A) site selection, mRNA cleavage, and adenosine residues polymerization. Besides the relevance of polyadenylation for gene expression, information is scarce in intestinal protozoan parasites that threaten human health. To better understand polyadenylation in Entamoeba histolytica , Giardia lamblia , and Cryptosporidium parvum , which represent leading causes of diarrhea worldwide, genomes were screened for orthologs of human factors. Results showed that Entamoeba histolytica and C. parvum have 16 and 12 proteins out of the 19 human proteins used as queries, respectively, while G. lamblia seems to have the smallest polyadenylation machinery with only six factors. Remarkably, CPSF30, CPSF73, CstF77, PABP2, and PAP, which were found in all parasites, could represent the core polyadenylation machinery. Multiple genes were detected for several proteins in Entamoeba , while gene redundancy is lower in Giardia and Cryptosporidium . Congruently with their relevance in the polyadenylation process, CPSF73 and PAP are present in all parasites, and CFIm25 is only missing in Giardia . They conserve the functional domains and predicted folding of human proteins, suggesting they may have the same roles in polyadenylation.