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Nbseptin2 Expression Pattern and Its Interaction with Nb PTP 1 during Microsporidia Nosema bombycis Polar Tube Extrusion
Author(s) -
Liu Fangyan,
Chen Jie,
Dang Xiaoqun,
Meng Xianzhi,
Wang Rong,
Bao Jialing,
Long Mengxian,
Li Tian,
Ma Qiang,
Huang Jun,
Pan Guoqing,
Zhou Zeyang
Publication year - 2019
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12752
Subject(s) - microsporidia , biology , spore , spore germination , microbiology and biotechnology , nosema , polar filament , germination , botany
Nosema bombycis ( Nb ) is a deadly species of microsporidia capable of causing pébrine, leading to heavy losses in sericulture. Germination is an important biological event in the invasion process of microsporidia. Septins, a family of membrane‐associated proteins, play a critical role in tissue invasion and have been recognized as a virulence factor in numerous pathogens. Previous work in our laboratory has shown that Nosema bombycis septin2 (Nbseptin2) interacts with subtilisin‐like protease 2 (Nb SLP 2). Herein, we found that Nbseptin2 was mainly associated with the plasma membrane in spores. Following spore germination, Nbseptin2 was found to co‐localize with polar tube protein 1 (Nb PTP 1) at the polar cap and proximal zone of the polar tube. Co‐immunoprecipitation and yeast two‐hybrid analysis further confirmed that Nbseptin2 interacted with Nb PTP 1. The translocation and interaction of Nbseptin2 in the spores suggest that Nbseptin2 may play a significant role in microsporidia polar tube extrusion process. Our findings improve understanding of the mechanisms underlying microsporidia germination.