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Influence of Heterologous Transplant of DNA ‐lacking Mitochondria from Entamoeba histolytica on Proliferation of Entamoeba invadens
Author(s) -
Kazama Makoto,
Yoshida Kazuhiro,
Ogiwara Sanae,
Makiuchi Takashi,
Tachibana Hiroshi
Publication year - 2018
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12693
Subject(s) - biology , entamoeba histolytica , heterologous , mitochondrial dna , microinjection , mitochondrion , entamoeba , dna , nuclear dna , microbiology and biotechnology , biochemistry , gene
In mitochondria, compatibility of proteins encoded in mitochondrial DNA and nuclear DNA is essential for the normal functioning of the organelle. Incompatibility between mitochondrial and nuclear DNA can lead to dysfunctional respiration, mitochondrial diseases, and lethal problems, which suggests that the presence of heterologous mitochondria is unfavorable. In a previous study, we established a transplant method for DNA ‐lacking mitochondria (mitosomes) in the anaerobic protozoan Entamoeba histolytica . In this study, interspecies transplant of mitosomes from E. histolytica into Entamoeba invadens , which is a parasitic protozoon of reptiles, was performed using the microinjection method at various temperatures and injection volumes. When E. invadens was used as recipient, it showed higher tolerance to a lower temperature and larger injection volume, in comparison with E. histolytica . After microinjection, donor mitosomes expressing HA ‐tag conjugated protein were observed in recipient cells by immunofluorescent staining. The heterologous mitosomes‐injected cells proliferated and growth rate of the microinjected‐cells was similar to that of intact cells. Therefore, we conclude that interspecies transplant of DNA ‐lacking mitochondria does not result in incompatibility.