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The Dynamics of Replication in Trypanosoma cruzi Parasites by Single‐Molecule Analysis
Author(s) -
de Araujo Christiane B.,
Calderano Simone G.,
Elias Maria Carolina
Publication year - 2018
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12676
Subject(s) - biology , trypanosoma cruzi , chromatin , dna replication , transcription (linguistics) , dna , dna polymerase , replication (statistics) , polymerase , genetics , microbiology and biotechnology , virology , parasite hosting , linguistics , philosophy , world wide web , computer science
Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although T. cruzi exhibits a mean fork speed (2.05 ± 0.10 kb/min) very similar to other trypanosomatids, we found that the majority of DNA molecules replicated more slowly, with a frequency distribution approximately 1 kb/min. This frequency distribution analysis provides more information about the replication profile of this organism.