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The Sanguicolous Apostome Metacollinia luciensis Jankowski 1980 (Colliniidae, Apostomatia, Ciliophora) Is Not Closely Related to Other Sanguicolous Apostomes
Author(s) -
Lynn Denis H.,
StrüderKypke Michaela C.
Publication year - 2018
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12638
Subject(s) - biology , phylogenetic tree , clade , phylogenetics , ribosomal rna , zoology , cytochrome c oxidase subunit i , gene , cytochrome c oxidase , crustacean , molecular phylogenetics , evolutionary biology , genetics , mitochondrion
The apostome family Colliniidae includes species that are adapted to the hemocoel/blood of various invertebrates, particularly crustaceans. To explore the phylogeny of these sanguicolous apostomes, Metacollinia luciensis was collected in August 2015 at Roscoff from the amphipod host, Orchestia gammarellus . Ciliates were Protargol stained and DNA was extracted. The small subunit rRNA ( SSU r RNA ) and cytochrome c oxidase subunit I ( cox 1) genes were amplified. Molecular phylogenetic analyses of the SSU r RNA genes unambiguously grouped M. luciensis with other apostomes with robust bootstrap support, but separated it distinctly from the pseudocolliniid clade. While there are only cox 1 sequences for a subset of these apostomes, M. luciensis was also distant from the pseudocolliniids and separated from them by species of the exuviotrophic apostome Hyalophysa . These results confirm the distinctness of the families Colliniidae and Pseudocolliniidae.