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Toxoplasma gondii Histone 4 Affects Some Functions of Murine Ana‐1 Macrophages In Vitro
Author(s) -
Liu Xinchao,
Li Xiaoyu,
Wang Qiangqiang,
Sun Xiaoni,
Lu Mingmin,
Ehsan Muhammad,
Xu Lixin,
Yan RuoFeng,
Song XiaoKai,
Li XiangRui
Publication year - 2018
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12630
Subject(s) - toxoplasma gondii , biology , histone , microbiology and biotechnology , in vitro , phagocytosis , antibody , immunology , dna , biochemistry
Toxoplasma gondii ( T. gondii ) is an obligate intracellular protozoan that can infect almost all nucleated cells. Histone proteins and DNA form the nucleosomes, which are the fundamental building blocks of eukaryotic chromatin. Histone 4 is an essential component of a histone octamer. In the present study, T. gondii histone 4 (TgH4) was cloned and the regulatory effect of TgH4 on murine macrophages was characterized. Bioinformatics analysis revealed that TgH4 was highly conserved in structure. Recombinant TgH4 ( rTgH 4) protein was identified by sera from rats experimentally infected with T. gondii and native TgH4 in the total soluble protein of T. gondii tachyzoites was recognized by polyclonal antibodies against rTgH 4, as indicated by immunoblotting analysis. Immunofluorescence assay showed that TgH4 binds to macrophages. Following incubation with rTgH 4, the toll‐like receptor 4 ( TLR 4) level of the macrophages was downregulated. Meanwhile, chemotaxis and the proliferation of macrophages were inhibited. However, rTgH 4 can promote phagocytosis, apoptosis, and the secretion of nitric oxide, interleukin‐6, and tumor necrosis factor‐α from macrophages. Just 80 μg/ ml rTgH 4 can significantly elevate the secretion of interleukin‐10 and interleukin‐1β ( p < 0.05 and p < 0.01). Viewed together, these outcomes indicated that rTgH 4 can affect the functions of murine macrophages in vitro.